Yaazh Xenomics offers reliable, high-accuracy Sanger DNA Sequencing services using the classic chain-termination method. As one of the most trusted genomics service providers in South India, we deliver precise nucleotide sequences with long read lengths up to 1000 bases per reaction — ideal for research, diagnostics, microbial identification, and validation studies.
Sanger sequencing, also known as the dideoxy chain termination method, remains the gold standard for DNA sequencing. Developed by Frederick Sanger, this technique uses dideoxynucleotides (ddNTPs) to terminate DNA synthesis at specific points, generating fragments of varying lengths that are separated by capillary electrophoresis to determine the exact order of nucleotides (A, T, C, G).
Despite the rise of Next-Generation Sequencing (NGS), Sanger sequencing continues to be preferred for its unmatched accuracy (99.99%), long reads, simplicity, and cost-effectiveness in small-to-medium scale projects.
Sanger sequencing is the method of choice when you need:
At Yaazh Xenomics, we combine cutting-edge capillary electrophoresis systems with proprietary protocols to deliver superior quality results:
We cater to high-quality sequencing of upto 1000 bases/reaction read length as opposed to 800 bases. We leverage cutting-edge and proprietary technology too.
We deliver consistent, solid data on templated like GC-rich areas, hairpins and more. With Yaazh Xenomics, you can always be assured of precise results.
We utilise trained team members with universal and free vector primers for DNA analysis. They have the expertise and experience to offer you reliable and accurate data.
We offer you a quick turnaround time, troubleshooting and free advice with each analysis. Whatever be the assistance you need, we will be happy to help.
The samples Yaazh uses for the Sanger DNA sequencing include plasmid, cosmid, genomic DNA, purified PCR products, un-purified PCR products, and single-stranded phage DNA.
We use chain termination PCR to make manifold copied of the DNA sequence under observation. Each oligonucleotide copy terminates at random length using a 5’-ddNTP.
We perform gel electrophoresis on the DNA samples. On application of a current, the negatively charged oligonucleotides move towards the other end of the gel matrix.
We read the gel to determine the DNA sequence of the sample. Because the synthesised DNA direction is 5’ to 3’, every last dideoxy-nucleotide correspond to a specific nucleotide in the original sample.
Contact our expert team today for a free consultation, custom quote, or sample submission guidelines.
Phone: +91 99431 32020 | +91 95002 45454
Address:
Module No. 103, TICEL BIOPARK Phase – III, 1st Floor,
Maruthamalai Road, Coimbatore - 641046, Tamil Nadu, India
Also serving clients from Visakhapatnam and across India.
Would you like to avail our expert Sanger DNA sequencing services? Talk to us for professional assistance.
Yaazh Xenomics is a leading biotechnology company based in Coimbatore, Tamil Nadu, India, specializing in comprehensive genomic solutions. As a DNA testing laboratory, we offer a broad spectrum of services, including DNA sequencing, RNA Sequencing, Sanger Sequencing, 16s rRNA, 18s rRNA, ITS, COI, RBCL, Matk gene Sequencing for DNA Barcoding, gene expression analysis, SNP analysis, Next-Generation Sequencing (NGS), Various Medical Genome testing, Exome Sequencing, Gut Microbiome Test, Metagenome Sequencing, Whole Genome Sequencing (WGS), Transcriptome Sequencing using advance NGS platforms like Nanopore, Illumina, MGI, Thermo. Also, we provide advance Bioinformatics, Customized Bioinformatics and a variety of other genetic testing and Molecular testing.