Decoding the rRNA Gene

Fragments of rDNA remain remarkably similar in organisms that are distantly related. Therefore, sequencing and comparing it can show evolutionary relatedness. It also helps measure the difference between organisms.

There are many ways to study it; 16s rRNA gene sequencing is the most common. At Yaazh, a true whole-genome sequencing lab, we help you understand rRNA to determine taxonomy and phylogeny. Our accurate results can help estimate the divergence rates in bacteria. 

We give you access to one of the most important tools for both actinomycetes and bacteria using universal primers 27F/1427R. We sequence with 518F/800R. We sequence, assemble and analyse. As part of our genomic services, we also construct the phylogenetic tree. 

Better and More Comprehensive Results

Our next-generation sequencing services are full-package, including DNA extraction and PCR amplification followed by gel verification and purification. 

Sequence raw data (Forward and Reverse) in different file formats.
.ab1 - Sequence with chromatogram
.pdf - Sequence with chromatogram
.txt - Only sequence
.fasta - Only Sequence
Assembled sequence (Contig) - ~ 1400bp or more
Blast result generated from all GenBank + NCBI+EMBL+DDBJ+PDB sequences.
Summary report comprises
Methodology for DNA Extraction, PCR, Sequencing and Bioinformatic analysis.
Sequence analysis report contains sequence read normal length, Q16 & Q20 read length, GC content details.
Proper contigs sequence with coverage image
Final result
Phylogeny tree.
We provide all the bioinformatics analysis, including the phylogenetic tree & customer-specific gene analysis with free of cost.

Customer-specific gene analysis and a phylogenetic tree are provided free of cost.

The Technique- A Complete Solution

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PCR amplification

After the bacteria is isolated and DNA extracted, we amplify the 16s rRNA sequence. We rely on 27F/1492R or 27F/1525R, the universal primers that complement the region. Once we have multiple copies of the target DNA, we utilise them as sequence templates. 

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DNA sequencing

This step uses the common Sanger sequence method. Each strand - 3' to 5' and 5' to 3' – undergoes separate sequencing. We use forward and reverse primers. Often they are the same universal primers from amplification. Internal primers are used when entire regions need to be sequenced. The common primers Yaazh wields for 16s rRNA are 518F/800R.

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Sequence comparison

The last stage is creating a phylogenetic tree using bioinformatic tools to visualise the result. The tree shows either the similarity or distance between the clustered calculation. It also helps compare sequences.

Power of Functional Genomics

The only whole-genome sequencing lab you will ever need for 16s rRNA sequencing services!
Get Started
Yaazh Xenomics,
Module No. 103,
1st floor, Maruthamalai Road,
Coimbatore - 641046.
Yaazh Xenomics is dedicated to supporting taxonomists’ research in the molecular identification of organisms using DNA barcoding markers, Sanger sequencing, and Next-Generation DNA sequencing techniques.
Copyright © 2023 Yaazh Xenomics. All Rights Reserved

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