Excellence In 18s rRNA Gene Sequencing

Yaazh Xenomics owns a high reputation for its extensive rRNA gene sequencing services that are a popular solution to identifying fungi and algae. We use ITS1, ITS2, ITS3, ITS4, and ITS5 primer combinations for amplifying the sequencing in the ITS region. The derived ITS sequence data is crucial in finding out the similarity of the given microorganism in various gene banks. The sequence data we derive also plays a vital role in calculating the similarity between the rRNA database and the target fungi or algae.

Our 18s rRNA gene sequencing services are the complete solutions for DNA extraction, PCR amplification, gel verification, purification, sequence assembling, bioinformatics analysis, and the phylogenetic tree's construction.

18s rRNA Sequencing: The Results

We process DNA samples, pure and impure PCR products to achieve possible results with
the shortest turnaround time.The results of our 18s rRNA sequencing include:

.ab1 - Sequence with chromatogram.
.pdf - Sequence with chromatogram.
.txt - Only sequence.
.fasta - Only Sequence.
Assembled Sequence (Contig).
Blast result generated from All GenBank.
NCBI+EMBL+DDBJ+PDB sequences.
Proper contigs sequence with a cover image.
Final Result
Phylogeny Tree.

How 18S rRNA Sequencing Sheds Light on Microeukaryote Diversity

Understanding the intricate dance of microeukaryote diversity in water systems is critical, especially regarding the impact of human activities. A powerful tool in this exploration is 18S rRNA sequencing. Here's how it contributes to our understanding:

  1. Detailed Profiling of Communities

    • 18S rRNA sequencing allows for precise identification of picoeukaryote communities, even distinguishing between rare and abundant taxa. This detailed profiling helps in recognizing how pollution influences these communities, paving the way for targeted environmental interventions.
  2. Detecting Environmental Impacts

    • By analyzing 18S rRNA sequences, researchers can observe how variations in environmental parameters, such as nutrient levels, salinity, and oxygen concentration, shape microeukaryote diversity. These insights reveal how alterations in these variables—often driven by human activities—affect ecosystem balance.
  3. Understanding Gradient Effects

    • This sequencing method helps track how human-generated contaminants in water systems cause shifts in community structures. By examining changes in species composition across ecological gradients, scientists gain insights into the influence of pollutants and other anthropogenic factors.
  4. Spotlighting Rare Taxa

    • One of the major contributions of 18S rRNA sequencing is its ability to spotlight rare subcommunities. This is crucial as it highlights lesser-known groups, such as those primarily found in zooplankton, which can act as indicators of environmental health.
  5. Holistic View of Ecosystem Dynamics

    • Beyond identifying specific species, 18S rRNA sequencing provides a comprehensive view of ecosystem dynamics, emphasizing the riverine ecosystems' resilience and adaptability, while underscoring the considerable impact of human. 
    • activities on their diversity.

In essence, 18S rRNA sequencing equips researchers with a high-resolution lens to view the ripple effects of human actions on microeukaryotic diversity, enabling more informed conservation and management strategies.

Why is 18S rRNA Used for Studying Eukaryotes?

18S rRNA plays a pivotal role in the study of eukaryotes due to its essential functions in cellular processes and its highly conserved nature. Here’s why it is commonly used:

In summary, the stability, functional importance, and evolutionary conservation of 18S rRNA make it an indispensable tool in eukaryotic research, providing invaluable insight into the genetic and structural nuances of these complex organisms.

Comparing 16S rRNA and 18S rRNA

When exploring the molecular world of ribosomal RNA, it's essential to understand the differences between 16S rRNA and 18S rRNA regarding cell types, functions, sequence lengths, and applications.

Cell Types

Functions

Sequence Length and Structure

Applications

In summary, while both 16S and 18S rRNA are critical to ribosome structure and function, they differ significantly in terms of the organisms they are associated with, the specifics of their roles, their genetic makeup, and the scientific and ecological applications they support.

Their article provides a comprehensive and detailed explanation of the differences between ITS and 18S sequencing regarding both target sequences and applications.

Target Sequences

Applications

 

Talk To Our Experts Today

Talk To Our Experts Today

Contact us today to know further details about our pricing for
18srRNA sequencing services.
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Yaazh Xenomics,
Module No. 103,
TICEL BIOPARK Phase – III,
1st floor, Maruthamalai Road,
Coimbatore - 641046.
Yaazh Xenomics is dedicated to supporting taxonomists’ research in the molecular identification of organisms using DNA barcoding markers, Sanger sequencing, and Next-Generation DNA sequencing techniques.
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