Real-Time Polymerase Chain Reaction (also known as quantitative PCR or qPCR) is a highly sensitive and specific technique that monitors the amplification of DNA or cDNA in real time using fluorescent reporters. Unlike conventional PCR, qPCR allows precise quantification of starting template amounts during the exponential phase, making it the gold standard for gene expression analysis, viral load determination, and pathogen detection.

Reverse Transcription qPCR (RT-qPCR) additionally converts RNA to cDNA, enabling quantification of mRNA, miRNA, and other RNA molecules.

Our Advanced RT-PCR / qPCR Capabilities

• Relative and Absolute Quantification
• Gene Expression Analysis (mRNA and miRNA)
• Transcript Variant Detection and Isoform Analysis
• Viral Load Quantification and Pathogen Detection
• Allelic Discrimination / SNP Genotyping
• miRNA Expression Profiling
• Validation of NGS / Microarray Data
• High-Throughput Screening of Bulk Samples
• Custom Assay Design and Optimization

Platforms & Chemistry We Use

We employ multiple detection chemistries and instruments to match your experimental needs:

• TaqMan Probe-Based Assays — High specificity and multiplexing capability for precise quantification
• SYBR Green Chemistry — Cost-effective option for gene expression studies with melt curve analysis
• Agilent & Affymetrix Platforms — Advanced real-time PCR systems for high-throughput applications
• In-house primer and probe synthesis for faster turnaround and optimized performance

End-to-End RT-PCR Workflow at Yaazh Xenomics

• High-quality RNA / DNA extraction from various sample types (tissues, blood, cells, microbes, plants, FFPE)
• RNA quality assessment (RIN / integrity check) and reverse transcription (when required)
• Primer / probe design, validation, and optimization
• Real-time PCR setup with appropriate controls (no-template, no-RT, positive controls)
• Data acquisition and analysis using advanced software (Ct value determination, standard curves, efficiency calculation, ΔΔCt method)
• Comprehensive reporting with graphs, statistics, and interpretation support

Key Applications of Our RT-PCR Services

• Gene expression studies under different conditions or treatments
• miRNA and non-coding RNA quantification
• Viral quantification (SARS-CoV-2, other pathogens)
• Pathogen detection and load monitoring
• Biomarker discovery and validation
• Allelic discrimination and genotyping
• cDNA template generation for cloning and sequencing
• Validation of RNA-Seq, microarray, or NGS results
• Agricultural research (plant stress response, GM detection)
• Clinical and diagnostic research

Why Choose Yaazh Xenomics for RT-PCR / qPCR Services?

• Highly experienced molecular biologists with expertise in assay optimization
• Robust, economical, and scalable workflow suitable for small to large sample batches
• Fast turnaround time with priority processing options
• Strict quality control and inclusion of all necessary controls
• Competitive pricing tailored for academic, biotech, pharma, and diagnostic clients
• Seamless integration with other services: Sanger Sequencing, NGS (RNA-Seq), Bioinformatics, and HPLC
• Personalized consultation and technical support from sample submission to data interpretation