Ruling as South India’s most advanced genomics laboratory does not come only from our sequencing services. Our lab provides every vital support required to probe into the vast field of genomics and support our clients in simple or complex research projects. We make this possible by offering molecular services that examine the fine nuances of genomics.
Molecular genomics is a critical component that adds crucial elements to the expanding DNA / RNA analysis database, their link with diseases, disease prognosis, and therapeutic response. At Yaazh Xenomics, we offer a slew of molecular services to support extensive clinical research to diagnose diseases to establish a connection to the patient’s genetic profile.
Our highly reliable transcript services provide invaluable post-research support with the finest proofreading and editing for research paper publication, PhD thesis, dissertation, and college projects.
We enhance the clarity and coherence of your thesis by working on the language style, structure, and presentation. Our thesis editing is what you need to enhance the marketing criteria of your thesis.
We enhance the clarity and coherence of your thesis by working on the language style, structure, and presentation. Our thesis editing is what you need to enhance the marketing criteria of your thesis.
We are a one-stop shop for expert support in protein and cell analysis, immunology, and molecular biology. Our collaboration with global leaders allows us to offer customised services for academic, clinical, and diagnostic research.
We deploy the finest molecular technology to develop DNA markers with Randomly Amplified Polymorphic DNA, a PCR-based technique. Our technique is a powerful method of analysis in detecting genetic diversity.
Yaazh offers in-depth AFLP techniques to assess genetic diversity within species, to deduce biogeographic patterns and phylogenies, generate genetic maps, and detect various polymorphisms.
We use restriction enzymes to create DNA fragments for Terminal Restriction Fragment Length Polymorphism. All DNA fragments are labelled with a fluorescent molecule and separated by Gel Electrophoresis.
Flip through international journals that delve deep into cellular, functional and molecular aspects of therapy. The journals help you understand the mechanisms clearly and detect the elements that can directly influence human health.
We offer you a comprehensive set of proteomic services that include peptide synthesis, protein purification, protein Identification, protein profiling, 2D electrophoresis, clinical protein electrophoresis, and protein expression.
PCR product analysis involves the examination and characterization of DNA fragments amplified through Polymerase Chain Reaction (PCR). This service is crucial for verifying amplification success, assessing product size and purity, and detecting specific genetic sequences, supporting various molecular biology applications.
Yaazh performs GMO detection by identifying specific genetic sequences introduced into organisms through genetic engineering. This service utilizes advanced molecular techniques to accurately detect the presence of genetically modified organisms in various samples, ensuring compliance and product integrity.
Restriction digest analysis is a molecular biology technique used to cut DNA at specific recognition sites using restriction enzymes. This analysis helps in DNA mapping, cloning, and identifying genetic variations, providing insights into DNA structure and function.
Gene expression analysis measures the activity of genes by quantifying the amount of RNA produced. This service helps researchers understand how genes are turned on or off in different conditions, crucial for studying disease mechanisms, drug responses, and biological processes.
Detection of low levels of RNA is performed using highly sensitive molecular techniques designed to accurately quantify minute amounts of RNA. This service is essential for studies involving rare transcripts, early disease detection, and samples with limited genetic material, ensuring precise and reliable results.
Analysis of total RNA involves assessing the quantity and quality of all RNA molecules present in a sample, including mRNA, rRNA, and small RNAs. This service is a critical first step for many downstream applications like gene expression profiling and sequencing, ensuring the integrity of genetic material for accurate results.
Analysis of Cy5 labelled samples involves detecting and quantifying molecules tagged with the Cy5 fluorescent dye. This service is commonly used in microarray experiments, fluorescence microscopy, and other molecular assays to visualize and measure specific biological components, providing high-resolution data.
Ribosomal RNA (rRNA) contamination in mRNA samples is detected and addressed through specialized depletion or removal techniques to ensure high-quality mRNA for downstream applications. This service is crucial because rRNA constitutes the majority of total RNA and can interfere with accurate gene expression analysis and sequencing results.
Analysis of RNA transcripts entails the study of individual RNA molecules produced from gene transcription, including their quantity, structure, and modifications. This service is vital for understanding gene regulation, alternative splicing, and the functional roles of different RNA species in biological processes and disease.
Optimisation of protein purification is achieved by refining experimental conditions and protocols to maximize the yield and purity of target proteins. This service involves evaluating various chromatography techniques, buffer conditions, and expression systems to ensure high-quality protein for structural and functional studies.
Protein expression is the process by which proteins are synthesized, modified, and regulated within living cells, and it is utilized to produce specific proteins for research, therapeutic, or industrial applications. This service involves selecting appropriate expression systems and optimizing conditions to achieve high yields of functional proteins.
Antibody analysis involves characterizing antibodies for their specificity, affinity, and concentration, crucial for their use in diagnostics, therapeutics, and research. This service employs various immunological techniques to ensure the quality and performance of antibodies for reliable experimental results.
Fast analysis of proteins between 5-50 kDa is performed using specialized techniques like capillary electrophoresis or rapid SDS-PAGE, designed for quick and efficient characterization of proteins within this specific molecular weight range. This service is valuable for rapid quality control, protein identification, and monitoring purification steps.
Analysis of protein purification involves assessing the purity, concentration, and integrity of proteins throughout the purification process. This service utilizes techniques such as SDS-PAGE, mass spectrometry, and spectrophotometry to ensure the successful isolation of target proteins for downstream applications.
Human and mammalian cell culture involves the growth and maintenance of cells in an artificial environment outside their natural habitat. This service is fundamental for a wide range of biological research, including drug discovery, disease modeling, and vaccine production, providing a controlled system for studying cellular processes.
Slide making for microscopic analysis involves preparing biological samples on glass slides for detailed examination under a microscope. This service includes fixation, embedding, sectioning, and staining techniques to preserve tissue morphology and highlight specific cellular components, essential for pathology and research.
Chromosome harvesting is a cytogenetic technique used to isolate chromosomes from dividing cells, typically for karyotyping and genetic analysis. This service involves culturing cells, arresting them at metaphase, and then preparing them on slides to visualize chromosomal structure and detect abnormalities.
Yaazh characterises tumour-related cell lines for chromosome rearrangement by employing advanced cytogenetic and molecular techniques to identify structural changes in chromosomes. This service is crucial for understanding cancer progression, identifying biomarkers, and developing targeted therapies, providing insights into the genetic basis of cancer.
Special studies using a variety of banding techniques, including G, C, and R, are employed to visualize distinct patterns on chromosomes, aiding in the identification of chromosomal abnormalities. These techniques are essential for detailed karyotyping, detecting subtle rearrangements, and understanding the structural integrity of chromosomes in genetic research and diagnostics.
Standard metaphase analysis with G-banding technique is a cytogenetic method used to identify and characterize chromosomes by creating unique banding patterns. This service is fundamental for detecting numerical and structural chromosomal aberrations, providing a comprehensive overview of an individual's karyotype for diagnostic and research purposes.
Potential DNA damage is assessed using various genotoxicity assays that detect alterations in DNA structure, such as breaks, adducts, or cross-links. This service is crucial for evaluating the genotoxic potential of chemicals, environmental factors, and therapeutic agents, providing insights into cellular health and disease risk.
Chromosomal aberrations such as breaks, gaps, large deletions, dicentrics, chromosomal fragmentations, and complex rearrangements are detected through advanced cytogenetic techniques like karyotyping and FISH. This service is essential for diagnosing genetic disorders, assessing genomic instability, and understanding the etiology of various diseases, including cancer.
Yaazh studies chromosomal instability or aberrations due to radiation or mutagen treatments by employing specialized cytogenetic assays to quantify and characterize induced genomic damage. This service is critical for genotoxicity testing, risk assessment, and understanding the biological impact of environmental exposures and therapeutic interventions on genomic integrity.
Assays used for Cell Viability (MTT), Cell Proliferation (BrdU), and Cell Survival (Bcl/Bax/NFKB) are crucial for evaluating cellular responses to various treatments and conditions. These services provide quantitative data on cell health, growth rates, and apoptotic pathways, essential for drug screening, toxicology, and basic research.
An Apoptosis Assay is a laboratory method used to detect and quantify programmed cell death, or apoptosis, in cell populations. This service is vital for studying disease mechanisms, drug efficacy, and cellular responses to stress, providing insights into cell fate and regulatory pathways.
A Cytotoxicity Assay is a laboratory test used to measure the toxic effects of compounds on cells, and methods include MMTT, TXT, MTS, NRU, and NHK-NRU. This service is essential for drug discovery, toxicology screening, and assessing the safety of various substances by quantifying cell death or inhibition of cell growth.
A Genotoxicity Assay is a laboratory test used to detect damage to genetic material within cells, and methods include SSCE, NOR, AgNOR, and COMET assays. This service is critical for assessing the mutagenic and carcinogenic potential of chemicals and environmental agents, ensuring safety and regulatory compliance.
Cell Cycle Progression is analyzed using markers like CDK2, CDK4, and p53 to monitor the orderly sequence of events by which a cell duplicates its contents and divides. This service is essential for understanding cell growth, differentiation, and the mechanisms underlying diseases like cancer, providing insights into cellular regulation.
The Brine Shrimp Lethality (in vivo) assay is a rapid and cost-effective bioassay used to determine the general toxicity of compounds by measuring their lethal effect on Artemia salina nauplii. This service serves as a preliminary screen for potential cytotoxic or pharmacological activities of natural products and synthetic compounds.
DPPH radical scavenging is an antioxidant assay that measures the ability of a compound to donate a hydrogen atom to stabilize the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical. This service is widely used to evaluate the antioxidant potential of natural extracts and pure compounds, indicating their capacity to neutralize free radicals.
Superoxide anion scavenging is measured using the PMS-NADH system, an assay that quantifies the ability of a substance to inhibit the formation or neutralize superoxide radicals. This service is important for assessing the antioxidant capacity of compounds against one of the most common reactive oxygen species, relevant in oxidative stress research.
ABTS radical scavenging is an antioxidant assay that measures the capacity of a compound to neutralize the ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical cation. This service is widely used to evaluate the total antioxidant capacity of various samples, including food, beverages, and biological fluids, providing a broad measure of antioxidant activity.
Nitric-oxide scavenging is an assay that assesses the ability of a compound to inhibit or neutralize nitric oxide radicals. This service is important for evaluating the anti-inflammatory and antioxidant properties of substances, as excessive nitric oxide production is implicated in various inflammatory and pathological conditions.
The ORAC (Oxygen Radical Absorbance Capacity) hydrophilic assay measures the antioxidant capacity of water-soluble compounds by quantifying their ability to inhibit the oxidative degradation of a fluorescent probe. This service is a widely recognized method for assessing the antioxidant potential of various samples, particularly those containing hydrophilic antioxidants.
Hydroxyl radical scavenging is an assay that measures the ability of a compound to neutralize hydroxyl radicals, which are highly reactive oxygen species known to cause severe cellular damage. This service is crucial for evaluating the protective effects of antioxidants against oxidative stress and related pathologies.
P70S6 kinase inhibition refers to the process of blocking the activity of p70S6 kinase, an enzyme crucial for cell growth, proliferation, and protein synthesis. This service is significant in cancer research and drug development, as inhibiting this kinase can suppress tumor growth and progression.
Glucose-6-Phosphatase inhibition refers to blocking the activity of the enzyme glucose-6-phosphatase, which plays a key role in glucose homeostasis by catalyzing the final step of gluconeogenesis and glycogenolysis. This service is relevant in diabetes research, as inhibiting this enzyme can help lower blood glucose levels.
Alpha-Glucosidase inhibition refers to blocking the activity of alpha-glucosidase enzymes, which are responsible for breaking down complex carbohydrates into absorbable glucose in the small intestine. This service is significant in managing type 2 diabetes, as inhibitors can reduce post-meal blood glucose spikes.
IKK-β (NF-κB kinase) inhibition refers to blocking the activity of IKK-β, a key enzyme in the NF-κB signaling pathway that regulates immune responses and inflammation. This service is highly relevant in developing anti-inflammatory and anti-cancer therapies, as NF-κB dysregulation is linked to many diseases.
PTP-1B inhibition refers to blocking the activity of Protein Tyrosine Phosphatase 1B, an enzyme that negatively regulates insulin signaling. This service is a significant area of research for developing new treatments for type 2 diabetes and obesity, as PTP-1B inhibitors can enhance insulin sensitivity.
Alpha-Amylase inhibition refers to blocking the activity of alpha-amylase, an enzyme responsible for breaking down complex carbohydrates into simpler sugars. This service is relevant in managing post-prandial hyperglycemia in diabetic patients, as inhibitors can slow down carbohydrate digestion and glucose absorption.
Malondialdehyde (MDA) inhibition refers to the process of reducing the formation or activity of MDA, a prominent biomarker of oxidative stress and lipid peroxidation. This service is crucial for evaluating the antioxidant and protective effects of compounds against cellular damage caused by reactive oxygen species.
Aldose Reductase inhibition refers to blocking the activity of aldose reductase, an enzyme involved in the polyol pathway, which is implicated in the development of diabetic complications. This service is important for research into preventing or mitigating conditions like diabetic neuropathy, retinopathy, and nephropathy.
Na+ - K+ - ATPase activity refers to the function of the sodium-potassium pump, a crucial enzyme responsible for maintaining ion gradients across cell membranes, vital for nerve impulse transmission and cellular volume regulation. This service is important for studying cellular physiology, drug effects on ion transport, and conditions like hypertension.
Anti-inflammatory / Anti Allergy Assays
Trypsin inhibition refers to blocking the activity of trypsin, a serine protease involved in protein digestion and various physiological processes. This service is relevant in studying protein-protein interactions, developing protease inhibitors for therapeutic purposes, and understanding digestive physiology.
Histamine release using RBL-2H3 cells is a common in vitro assay to study allergic reactions and mast cell degranulation. This service measures the amount of histamine released from RBL-2H3 mast cells upon stimulation, providing insights into potential anti-allergic compounds and mechanisms of hypersensitivity.
Nitric Oxide scavenging is an assay that assesses the ability of a compound to inhibit or neutralize nitric oxide radicals. This service is important for evaluating the anti-inflammatory and antioxidant properties of substances, as excessive nitric oxide production is implicated in various inflammatory and pathological conditions.
HRBC membrane stabilisation refers to the ability of a compound to protect human red blood cell (HRBC) membranes from lysis induced by hypotonic solution or heat. This service is used as an in vitro model to assess the anti-inflammatory and membrane-protective properties of various substances, indicating their potential therapeutic benefits.
15-Lipoxygenase inhibition refers to blocking the activity of 15-lipoxygenase, an enzyme involved in the metabolism of polyunsaturated fatty acids and the production of inflammatory mediators. This service is relevant in research for anti-inflammatory drugs and treatments for conditions like asthma and atherosclerosis.
PLA2 inhibition refers to blocking the activity of Phospholipase A2 (PLA2), an enzyme that plays a critical role in inflammation by releasing arachidonic acid from cell membranes. This service is significant in developing anti-inflammatory drugs, as PLA2 inhibitors can reduce the production of pro-inflammatory mediators.
LTB4 release refers to the cellular secretion of Leukotriene B4, a potent inflammatory mediator derived from arachidonic acid. This service is measured in studies of inflammation and immune responses, providing insights into the mechanisms of inflammatory diseases and the efficacy of anti-inflammatory agents.
Elastin release using IMR-90 cells is an assay that quantifies the secretion of elastin, a key protein responsible for the elasticity of tissues, from IMR-90 human lung fibroblasts. This service is important for studying tissue remodeling, wound healing, and diseases involving extracellular matrix degradation, such as emphysema.
Hyaluronidase inhibition refers to blocking the activity of hyaluronidase, an enzyme that degrades hyaluronic acid, a major component of the extracellular matrix. This service is relevant in research for anti-inflammatory, anti-aging, and anti-cancer therapies, as hyaluronidase activity is linked to tissue degradation and tumor invasion.
Elastase inhibition refers to blocking the activity of elastase, a protease that degrades elastin and other extracellular matrix proteins. This service is crucial in research for treating inflammatory diseases like emphysema and cystic fibrosis, where excessive elastase activity contributes to tissue damage.
Collagenase inhibition refers to blocking the activity of collagenase, an enzyme that breaks down collagen, the most abundant protein in the extracellular matrix. This service is significant in research for treating conditions involving excessive collagen degradation, such as arthritis, periodontitis, and certain cancers.
Collagen release using NIH-3T3 cells is an assay that quantifies the secretion of collagen, a primary structural protein, from NIH-3T3 mouse embryonic fibroblast cells. This service is important for studying extracellular matrix remodeling, wound healing, fibrosis, and the effects of various compounds on collagen synthesis and degradation.
Time-Kill kinetics is a microbiological assay that measures the rate and extent of microbial killing by an antimicrobial agent over time. This service is crucial for evaluating the bactericidal or fungicidal activity of new drugs, disinfectants, and preservatives, providing dynamic insights into their effectiveness.
The Resazurin Assay (Microtitre-plate) is a colorimetric and fluorometric assay used to assess cell viability and proliferation in a high-throughput format. This service measures the metabolic activity of cells by the reduction of resazurin to resorufin, providing a sensitive and non-toxic method for drug screening and cytotoxicity testing.
Anti-Bacterial / Fungal Susceptibility (MIC50 or MIC90) is determined by measuring the minimum inhibitory concentration (MIC) of an antimicrobial agent required to inhibit the growth of 50% or 90% of a microbial population. This service is essential for guiding antibiotic therapy, developing new antimicrobial drugs, and monitoring resistance patterns.
An Antibiotic susceptibility test or Antibiogram is a laboratory procedure used to determine the effectiveness of various antibiotics against a specific bacterial strain. This service is crucial for clinicians to select the most appropriate antibiotic treatment for infections, thereby improving patient outcomes and combating antibiotic resistance.
The Post-Antibiotic Effect (PAE) is the persistent suppression of bacterial growth that occurs after a short exposure to an antibiotic, even when the drug concentration falls below the minimum inhibitory concentration. This service is important for optimizing antibiotic dosing regimens and understanding the pharmacodynamics of antimicrobial agents.
Yaazh Xenomics is a leading biotechnology company based in Coimbatore, Tamil Nadu, India, specializing in comprehensive genomic solutions. As a DNA testing laboratory, we offer a broad spectrum of services, including DNA sequencing, RNA Sequencing, Sanger Sequencing, 16s rRNA, 18s rRNA, ITS, COI, RBCL, Matk gene Sequencing for DNA Barcoding, gene expression analysis, SNP analysis, Next-Generation Sequencing (NGS), Various Medical Genome testing, Exome Sequencing, Gut Microbiome Test, Metagenome Sequencing, Whole Genome Sequencing (WGS), Transcriptome Sequencing using advance NGS platforms like Nanopore, Illumina, MGI, Thermo. Also, we provide advance Bioinformatics, Customized Bioinformatics and a variety of other genetic testing and Molecular testing.