RNA-Seq is a powerful Next-Generation Sequencing (NGS) technique that sequences the entire transcriptome — all RNA molecules present in a cell or tissue at a given time. Unlike microarrays, RNA-Seq offers:

• Unbiased detection of known and novel transcripts
• High sensitivity and dynamic range for low-abundance transcripts
• Precise quantification of gene expression levels
• Discovery of alternative splicing events, fusion genes, and non-coding RNAs

Types of Transcriptome Sequencing Services We Provide

1. mRNA Sequencing (Poly-A Selected RNA-Seq)

Focused on protein-coding messenger RNA. Ideal for studying gene expression in eukaryotic samples. We use oligo-dT enrichment for high-quality mRNA libraries, delivering excellent coverage of coding regions with reduced ribosomal RNA background.

2. Total RNA Sequencing (rRNA Depleted)

Captures the complete transcriptome including mRNA, lncRNA, circRNA, and other non-coding RNAs. Recommended when you want to study both coding and non-coding RNA regulation without bias toward poly-A tails.

3. Small RNA / miRNA Sequencing

Targeted sequencing of microRNAs (miRNAs), siRNAs, and other small non-coding RNAs (18–30 nt). Essential for understanding post-transcriptional gene regulation, biomarker discovery, and disease mechanisms.

4. Long Non-Coding RNA (lncRNA) Sequencing

Specialized library preparation and deep sequencing to profile long non-coding RNAs (>200 nt) that play critical roles in gene regulation, chromatin modification, and disease progression.

5. Circular RNA (circRNA) Sequencing

Ribosomal RNA depletion combined with RNase R treatment for specific enrichment and detection of circular RNAs — important regulators in cancer, neurological disorders, and development.

6. Single-Cell RNA Sequencing (scRNA-Seq)

High-resolution transcriptomic profiling at the single-cell level. Reveals cellular heterogeneity, rare cell populations, and cell-type-specific gene expression patterns using 10x Genomics-compatible workflows or plate-based methods.

Our Advanced RNA-Seq Workflow

• High-quality RNA extraction and integrity assessment (RIN score evaluation)
• Library preparation with strand-specific chemistry and unique molecular identifiers (UMIs) for accurate quantification
• Sequencing on Illumina NovaSeq and other high-throughput platforms with flexible read depths (20M – 100M+ reads per sample)
• Comprehensive bioinformatics analysis including quality control, alignment (HISAT2/STAR), quantification (featureCounts/Salmon), differential expression (DESeq2/edgeR), GO & KEGG pathway enrichment, splicing analysis, and novel transcript discovery
• Delivery of raw data (FASTQ), processed results, interactive reports, and publication-ready figures

Key Applications of Transcriptome Sequencing

• Differential gene expression analysis under different conditions or treatments
• Identification of disease biomarkers and therapeutic targets
• Alternative splicing and isoform discovery
• Non-coding RNA regulatory networks (miRNA, lncRNA, circRNA)
• Single-cell transcriptomics for developmental biology and cancer research
• Host-pathogen interaction studies
• Plant stress response and crop improvement research
• Drug response and pharmacotranscriptomics
• Toxicology and environmental genomics

Why Researchers Choose Yaazh Xenomics for RNA-Seq Services

• Expertise in handling low-input and degraded RNA samples (FFPE, plasma, exosomes)
• High sequencing depth and data quality with strict quality control at every step
• Advanced bioinformatics pipelines with customized analysis as per your research goals
• Fast turnaround time — from sample to report in 4–6 weeks (depending on project size)
• Competitive pricing tailored for academic, biotech, and clinical researchers
• Seamless integration with other services: Whole Genome Sequencing, Metagenomics, Sanger validation, and qPCR
• Pan-India support with dedicated project management