Epigenome Sequencing Services
Decoding Epigenetic Regulation with NGS. Investigate heritable changes in gene expression that do not involve alterations in the underlying DNA sequence.
What is Epigenome Sequencing?
The epigenome consists of chemical modifications and proteins that regulate gene expression without changing the DNA sequence itself. Epigenome sequencing (epigenomics) uses NGS to map these modifications genome-wide, revealing how they control gene expression in health and disease.
<strong>Epigenetic mechanisms include:</strong>
• DNA methylation
• Histone modifications
• Chromatin accessibility
• Non-coding RNA
Explore Epigenetic Changes In Your Samples
Contact our epigenomics specialists for a free consultation, project design recommendations, or a customized quote.
Our Comprehensive Epigenome Services
WGBS
Whole Genome Bisulfite Sequencing. The gold-standard method for single-base resolution DNA methylation profiling across the entire genome.
RRBS
Reduced Representation Bisulfite Sequencing. Enriches CpG-rich regions for high coverage at lower sequencing costs.
ChIP-Seq
Chromatin Immunoprecipitation Sequencing. Maps genome-wide protein-DNA interactions and histone modifications.
ATAC-Seq
Fast, sensitive method to profile open chromatin regions using Tn5 transposase to identify active regulatory elements.
Advanced Workflow
- <strong>1.</strong> High-quality DNA/chromatin extraction from diverse samples (cells, tissues, FFPE, blood, plants, microbes).
- <strong>2.</strong> Optimized library preparation with bisulfite conversion, immunoprecipitation, or tagmentation.
- <strong>3.</strong> High-throughput sequencing on Illumina NovaSeq and Nanopore platforms with appropriate read depth.
- <strong>4.</strong> Comprehensive bioinformatics: Alignment (Bismark, Bowtie2), peak calling (MACS2), differential analysis, motif discovery, and visualization.
Applications
✓: Cancer epigenetics and biomarker discovery
✓: Developmental biology and stem cell research
✓: Neurological disorders and imprinting diseases
✓: Environmental epigenomics and toxicology
✓: Plant epigenetics for stress response and crop improvement
✓: Drug response prediction and pharmacoepigenomics
✓: Infectious disease research (host-pathogen epigenetic interactions)
✓: Aging and regenerative medicine studies