DNA Sequencing
(Sanger Sequencing & NGS)

Sanger sequencing (gold-standard for targeted/long-read accuracy), difficult template sequencing (high GC, repeats, hairpins), 96-well plate high-throughput sequencing, PCR product/plasmid/BAC sequencing, and hybrid approaches

Next-Generation Sequencing (NGS) platforms like Illumina, Oxford Nanopore, MGI, and Thermo – supporting research in plants, animals, microbes, human genetics, forensics, and beyond. With NGS Whole genome, Metagenome, Transcriptome, Whole Exome, Clinical Exome, Cancer Panels, Tageted Panels

Trusted by students, researchers, pharma, biotech, and diagnostic labs across India and internationally, we guarantee accuracy, rapid turnaround, cost-effective solutions, and publication-ready data with expert bioinformatics support.

What is DNA Sequencing?

DNA sequencing determines the precise order of nucleotide bases (A, T, C, G) in a DNA molecule – the fundamental "code" of life. This enables understanding genetic variation, function, evolution, disease mechanisms, and personalized applications.

Our DNA Sequencing Methods & Technologies

Sanger Sequencing – Gold Standard for Precision

Sanger (chain-termination) sequencing remains the reference method for targeted, high-accuracy reads up to 1000+ bases per reaction. Ideal for validation, single-gene analysis, microbial ID, and custom projects.

  • Forward + reverse reads for contig assembly and double-strand coverage
  • High success on standard PCR products, plasmids, BACs, clones
  • Proprietary optimizations for difficult templates (high GC/AT-rich, inverted repeats, long mono/di-nucleotide stretches)

96-Well Plate High-Throughput Sequencing

Economical batch processing for multiple samples – accept impure PCR products, purified DNA, or clones. Options: single primer per plate, dual primers, or multi-primer setups for flexibility and cost savings.

Hybrid & Long-Read DNA Sequencing

Combine Sanger precision with NGS scale: short-read (Illumina) for depth + long-read (Nanopore) for structural resolution, phasing, and complex regions.

End-to-End DNA Sequencing Workflow at Yaazh Xenomics

  1. Consultation & Design – Free guidance on method (Sanger vs. NGS), primer design, template prep, and project goals
  2. Sample Receipt & QC – DNA extraction/purification if needed; concentration, purity (A260/A280), integrity checks
  3. PCR Amplification (if required) – Optimized conditions + gel verification
  4. Sequencing – Sanger on capillary electrophoresis or NGS platforms; real-time basecalling for Nanopore
  5. Bioinformatics Analysis (Free basic + custom):
    • Trace viewing (.ab1), contig assembly, quality scores (Phred Q20+)
    • BLAST alignment, variant calling, phylogenetic trees
    • Custom reports: FASTA, chromatograms, summary PDF
  6. Delivery & Support – Raw files + interpreted results; post-delivery consultation

Sanger vs. NGS: Which DNA Sequencing Method for Your Project?

Sanger Sequencing Advantages

  • Highest per-base accuracy (~99.99%)
  • Long individual reads (up to 1000+ bp)
  • Cost-effective for low-volume, targeted sequencing (1–96 samples)
  • Fast TAT (often 1–3 days)
  • Gold standard for validation & clinical confirmation

When to Choose NGS Over Sanger

  • High-throughput needs (>100 targets/samples)
  • Whole-genome, exome, or deep variant detection
  • Cost per base lower for large-scale projects

For most targeted or validation work in India, Sanger remains the efficient choice – we handle both seamlessly.

Applications of Our DNA Sequencing Services

  • Microbial identification & barcoding (16S/18S rRNA, COI, ITS, rbcL, matK)
  • Plant/animal species authentication & phylogeny
  • Human genetics & rare variant confirmation
  • Forensics, paternity, & identity testing
  • Plasmid/clone verification in biotech R&D
  • Pathogen tracking & outbreak studies

Why Researchers Choose Yaazh Xenomics for DNA Sequencing in India

  • Affordable pricing – best value for students & large projects
  • Expert handling of difficult templates – high success rates
  • Fast, reliable turnaround from Coimbatore-based ISO lab
  • Nationwide support + international shipping
  • Free basic bioinformatics + publication assistance
01
DNA Sequencing

DNA Sequencing is the process used to determine the nucleotide's order or sequence in the DNA fragment. Discovered and developed by the scientist Frederick Sanger, the sequencing is performed using the chain termination method. This technique uses two strands of DNA that are sequenced separately for generating both forward and reverse sequences.

02
96 Well Plate Sequencing

The 96 well plate sequencing is most affordable and convenient for processing many samples that can be sent as impure PCR products, clones, and purified DNA. Plate sequencing service can be done as one or two primers per plate or multi primers per plate. 

03
Difficult Template Sequencing

Yaazh Xenomics' uniquely advanced technology solves Difficult Template Sequencing when standard shotgun protocols fail to deliver satisfactory results. The "Difficult Template" refers to the GC and AT-rich samples, multiple inverted hairpin repeats, as well as long simple mono and di-nucleotide repeats.

Get Your DNA Sequencing Project Started in India

Ready for precise, cost-effective DNA sequencing? Contact our Coimbatore team for a free consultation or quote today.

Yaazh Xenomics Pvt Ltd
Module No. 103, TICEL BIOPARK Phase – III,
Maruthamalai Road, Coimbatore – 641046, Tamil Nadu, India
(Also serving from Visakhapatnam, Andhra Pradesh)

Phone: +91 99431 32020 | +91 95002 45454
Email: info@yaazhxenomics.com

Request Quote | Sanger Sequencing Details | Explore NGS Options

Yaazh Xenomics – Accurate DNA Sequencing at the Best Prices: Decode Genetics with Confidence in India.

Contact Us
Yaazh Xenomics,
Module No. 103,
TICEL BIOPARK Phase – III,
1st floor, Maruthamalai Road,
Coimbatore - 641046.
Tamil Nadu, India
Yaazh Xenomics,
No.9-6, Sritej Nagar,
Anandapuram, 
Visakhapatnam -531022
Andhra Pradesh

Yaazh Xenomics is a leading biotechnology company based in Coimbatore, Tamil Nadu, India, specializing in comprehensive genomic solutions. As a DNA testing laboratory, we offer a broad spectrum of services, including DNA sequencing, RNA Sequencing, Sanger Sequencing, 16s rRNA, 18s rRNA, ITS, COI, RBCL, Matk gene Sequencing for DNA Barcoding, gene expression analysis, SNP analysis, Next-Generation Sequencing (NGS), Various Medical Genome testing, Exome Sequencing, Gut Microbiome Test, Metagenome Sequencing, Whole Genome Sequencing (WGS), Transcriptome Sequencing using advance NGS platforms like Nanopore, Illumina, MGI, Thermo. Also, we provide advance Bioinformatics, Customized Bioinformatics and a variety of other genetic testing and Molecular testing.

 

Copyright © 2026 Yaazh Xenomics. All Rights Reserved
| Designed by DigitalSEO | Sitemap
Top envelopephone-handsetmap-markermenucross-circletext-align-justify linkedin facebook pinterest youtube rss twitter instagram facebook-blank rss-blank linkedin-blank pinterest youtube twitter instagram