Gas Chromatography-Mass Spectrometry (GC-MS) combines gas chromatographic separation with mass spectrometric detection for high-resolution analysis of volatile/semi-volatile organics. Compounds are vaporized, separated on a capillary column, ionized (typically EI), and fragmented to produce unique mass spectra for identification and quantification.

Key strengths: Excellent sensitivity (ppb–ppt range), structural information from fragmentation patterns, and large databases for reliable unknown matching – making GC-MS the gold standard for volatiles where LC-MS is less suitable.

Our GC-MS Capabilities & Instrumentation

• Agilent or equivalent GC-MS systems with EI ionization (70 eV)
• Headspace (static/dynamic) & direct liquid injection sampling
• Columns: Non-polar (DB-5ms), mid-polar (DB-35ms), polar (DB-WAX) for diverse volatiles
• Scan/SIM modes for full scan identification & targeted quantification
• Method development/validation: Specificity, linearity, accuracy, precision, LOD/LOQ, recovery, robustness per USP/ICH/EPA guidelines
• Spectral matching against NIST/Wiley libraries + custom databases

End-to-End GC-MS Analysis Workflow

1. Consultation & Method Selection – Free expert advice on sample type (solvents, soil, water, pharma excipients), target analytes, and regulatory needs
2. Sample Preparation & QC – Headspace vial equilibration, SPME, liquid-liquid extraction, derivatization if needed; matrix-matched standards
3. Method Development (if required) – Column/oven program optimization, split/splitless injection tuning, MS parameters
4. Analysis & Validation – System suitability, calibration (multi-level), QC samples, full validation reporting
5. Data Processing & Reporting – Chromatogram/mass spectra review, library matching, quantification (internal/external standard), unknown identification; comprehensive report with raw data, spectra, conclusions

Why Choose Yaazh Xenomics for GC-MS Services in India?

• Affordable pricing – competitive for pharma QC, environmental labs, academia
• Regulatory compliance – USP <467> residual solvents, ICH Q3C, EPA methods
• High success on complex/unknown samples – extensive library expertise
• Fast TAT – efficient for release testing, stability volatiles
• ISO-certified lab in Coimbatore – strict QA, contamination controls, nationwide shipping
• Integrated with HPLC/LC-MS/NMR for full characterization

Key Applications of Our GC-MS Analysis

• Pharmaceuticals – Residual solvents (Class 1–3), process impurities, leachables/extractables
• Food & Beverages – Pesticide residues, flavor volatiles, contaminants (e.g., PAH, phthalates)
• Environmental – VOCs in water/soil/air, BTEX, organochlorine pesticides
• Forensic & Drug Detection – Volatile drugs, accelerants, poisons
• Herbal & Essential Oils – Volatile profiling, adulteration detection
• Unknown Identification – Mystery samples, off-odor analysis, polymer degradation

GC-MS Advantages & Limitations

Advantages

• Superior sensitivity & specificity for volatiles/semi-volatiles
• Robust library matching for unknowns
• Quantitative precision for regulatory compliance

Limitations

• Limited to volatile/thermally stable compounds (non-volatiles need derivatization)
• Matrix interference in complex samples (mitigated by cleanup/headspace)
• Requires careful sample prep to avoid column contamination

For non-volatile or polar analytes, explore our LC-MS or HPLC services.