HPLC is a powerful analytical technique used to separate, identify, and quantify components in a mixture. It works on the principle of differential partitioning between a stationary phase (column) and a mobile phase (solvent). HPLC offers superior resolution, sensitivity, and reproducibility compared to traditional chromatography, making it the gold standard for pharmaceutical quality control and research.

Our HPLC Capabilities

Instrumentation

• Agilent & Shimadzu HPLC systems with Quaternary pumps
• UV-Vis and Photodiode Array (PDA) detectors for multi-wavelength analysis
• Refractive Index (RI) and Fluorescence detectors
• Auto-samplers with temperature control
• Wide range of columns: C18, C8, Phenyl, Amino, Cyano, HILIC, Size-Exclusion, and Ion-Exchange

Key Services Offered

• Pharmaceutical Assay & Content Uniformity – Accurate quantification of Active Pharmaceutical Ingredients (API) and finished products
• Related Substances & Impurity Profiling – Detection and quantification of process impurities, degradation products, and genotoxic impurities
• Stability-Indicating Method Development & Validation – Forced degradation studies (acid, base, oxidative, thermal, photolytic) as per ICH Q1A-Q1E
• Method Development & Validation – Full validation as per ICH Q2(R1) including specificity, linearity, accuracy, precision, LOD, LOQ, robustness, and system suitability
• Dissolution Testing – Immediate and extended release formulations
• Herbal & Nutraceutical Analysis – Marker compound quantification and adulteration detection
• Residual Solvent Analysis – Complementary to GC-MS (Class 1, 2 & 3 solvents)

Our HPLC Workflow

1. Consultation & Method Selection – Understanding analyte properties, matrix complexity, and regulatory requirements
2. Sample Preparation – Optimized extraction, filtration, and dilution protocols for complex matrices
3. Method Development – Systematic screening of mobile phase, column, gradient, and detector parameters
4. Method Validation – Rigorous testing for specificity, linearity (1/x or 1/x² weighting), accuracy, precision, robustness, and solution stability
5. Routine Analysis & Reporting – System suitability testing, multi-level calibration, QC samples, and comprehensive report with chromatograms, calculations, and conclusions

Applications of Our HPLC Analysis Services

• Pharmaceutical Quality Control & Release Testing
• Stability Studies for Drug Substances and Products
• Bioequivalence and Bioavailability Studies
• Herbal Product Authentication and Standardization
• Food Additive and Preservative Analysis
• Environmental Monitoring of Pesticides and Pollutants

Why Choose Yaazh Xenomics for HPLC Analysis?

• Experienced analytical team with deep expertise in method development and validation
• Modern HPLC systems with high sensitivity and reproducibility
• Regulatory-compliant documentation for submissions (ANDA, NDA, DMF)
• Fast turnaround time for routine and urgent projects
• Affordable pricing with transparent packages
• ISO-certified laboratory with strict quality assurance