18S rRNA Sequencing Services in India
Eukaryotic Microbial ID & Diversity Analysis. Accurate, publication-ready identification of fungi, protists, algae, and micro-eukaryotes for researchers and industries.
What is 18S rRNA Sequencing?
While 16S rRNA is used for identifying bacteria, the 18S ribosomal RNA (rRNA) gene is the standard marker for eukaryotic organisms. It contains both highly conserved regions (for designing universal primers) and hypervariable regions (V4, V5, V9) that allow for precise taxonomic classification of micro-eukaryotes.
Yaazh Xenomics provides end-to-end 18S rRNA sequencing services using high-throughput NGS (Illumina / Oxford Nanopore) and gold-standard Sanger sequencing to deliver precise insights into eukaryotic communities.
Key Benefits of 18S rRNA Sequencing
High-Resolution Taxonomy: Identify micro-eukaryotes down to the genus/species level.
Culture-Independent: Sequence complex environmental samples without isolating individual species.
Broad Range: Ideal for studying fungi, algae, protists, and nematodes.
End-to-End Bioinformatics: From raw data to publication-ready diversity reports (Alpha/Beta diversity, PCoA).
Fast Turnaround: Results delivered in 15-20 days for NGS.
Workflow / Steps
Sample Collection & QC
Checking sample integrity and providing collection kits.
DNA Extraction
High-yield extraction from Soil, Water, Feces, and Tissue.
Target Amplification
Amplification of V4-V5 hypervariable regions.
NGS Sequencing
Illumina NovaSeq or Oxford Nanopore for high-depth coverage.
Bioinformatics Analysis
Processing via QIIME2, using SILVA/PR2 databases.
Publication-Ready Reports
Taxonomy tables, Alpha/Beta Diversity, PCoA, Phylogeny.
Contamination Control & Coverage in 18S Analysis
Improving Coverage
Eukaryotic DNA is often dwarfed by bacterial DNA in environmental samples. We optimize PCR conditions and sequencing depth to ensure rare eukaryotic taxa are not missed.
Contamination Mitigation
- Strict clean-room DNA extraction.
- Host DNA depletion (if required for clinical samples).
- Automated filtering of non-target (bacterial/archaeal) sequences.
Pros & Cons of 18S rRNA Sequencing
Advantages
- ✓ Excellent for studying overall eukaryotic community structure and diversity.
- ✓ Well-established databases (SILVA, PR2).
- ✓ Cost-effective for large-scale microbiome studies.
Limitations
- ✗ Generally lacks the resolution to differentiate closely related fungal species. (For high-resolution fungal identification, ITS Sequencing is recommended).
- ✗ Cannot identify bacteria or viruses.
- ✗ Does not provide functional data (What are the eukaryotes doing?).
If you require deeper species-level resolution for fungi, explore our ITS Sequencing Services
Applications of 18S rRNA Sequencing
- •Environmental Monitoring (marine, freshwater, and soil eukaryotes)
- •Agricultural Research (plant-pathogen interactions)
- •Gut Microbiome Studies (parasites and commensal fungi)
- •Harmful Algal Bloom monitoring
- •Aquaculture health and pathogen detection
Start Your 18S Eukaryotic Diversity Study
Get comprehensive, publication-ready microbiome reports with Yaazh Xenomics.