Yaazh Xenomics offers reliable, affordable 16S rRNA sequencing in India for precise bacterial and archaeal identification, microbial diversity studies, and phylogenetic analysis. As a leading genomics lab in Coimbatore, Tamil Nadu, we provide end-to-end service – from DNA extraction to publication-ready phylogenetic trees – helping researchers, microbiologists, and industries across India.
Trusted 16S rRNA gene sequencing lab delivering high-quality results for environmental, clinical, food safety, agriculture, and research samples.
The 16S ribosomal RNA gene is highly conserved yet contains variable regions unique to different bacteria and archaea. Sequencing this ~1500 bp gene allows accurate taxonomic classification, species-level identification in many cases, and construction of phylogenetic trees to understand evolutionary relationships.
At Yaazh Xenomics, we use universal primers (27F/1492R or similar) + Sanger sequencing (with 518F/800R internal) for gold-standard accuracy on pure isolates or simple communities. For complex microbiomes, we guide toward our NGS metagenome options.
High coverage ensures detection of rare species and accurate diversity estimates. We optimize read length & quality; rarefaction guidance available for complex samples.
For functional profiling or strain-level resolution, explore our shotgun metagenome sequencing.
Ready for precise microbial identification? Contact Coimbatore’s trusted genomics partner today.
Yaazh Xenomics Pvt Ltd
Module No. 103, TICEL BIOPARK Phase – III,
Maruthamalai Road, Coimbatore – 641046, Tamil Nadu
Phone: +91 99431 32020 | +91 95002 45454
Email: info@yaazhxenomics.com
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Yaazh Xenomics – Crack the Microbial Code with Precision & Affordability.
After the bacteria is isolated and DNA extracted, we amplify the 16s rRNA sequence. We rely on 27F/1492R or 27F/1525R, the universal primers that complement the region. Once we have multiple copies of the target DNA, we utilise them as sequence templates.
This step uses the common Sanger sequence method. Each strand - 3' to 5' and 5' to 3' – undergoes separate sequencing. We use forward and reverse primers. Often they are the same universal primers from amplification. Internal primers are used when entire regions need to be sequenced. The common primers Yaazh wields for 16s rRNA are 518F/800R.
The last stage is creating a phylogenetic tree using bioinformatic tools to visualise the result. The tree shows either the similarity or distance between the clustered calculation. It also helps compare sequences.
Yaazh Xenomics is a leading biotechnology company based in Coimbatore, Tamil Nadu, India, specializing in comprehensive genomic solutions. As a DNA testing laboratory, we offer a broad spectrum of services, including DNA sequencing, RNA Sequencing, Sanger Sequencing, 16s rRNA, 18s rRNA, ITS, COI, RBCL, Matk gene Sequencing for DNA Barcoding, gene expression analysis, SNP analysis, Next-Generation Sequencing (NGS), Various Medical Genome testing, Exome Sequencing, Gut Microbiome Test, Metagenome Sequencing, Whole Genome Sequencing (WGS), Transcriptome Sequencing using advance NGS platforms like Nanopore, Illumina, MGI, Thermo. Also, we provide advance Bioinformatics, Customized Bioinformatics and a variety of other genetic testing and Molecular testing.