UPLC Analysis Services In India
High-Resolution Ultra Performance Liquid Chromatography for robust analytical testing.
What is UPLC?
UPLC (Ultra Performance Liquid Chromatography) is an advanced liquid chromatography technique that utilizes columns packed with particles less than 2 µm in diameter. This allows operation at much higher pressures than traditional HPLC (up to 15,000 psi).
Key Advantages
3–10× faster analysis time:
2–5× higher resolution and peak capacity:
Improved sensitivity and reduced solvent consumption:
Better separation of complex mixtures:
Workflow / Steps
Project Consultation
Understanding regulatory requirements, analyte properties, and matrix challenges
Sample Preparation
Optimized extraction and clean-up procedures for complex matrices
Method Development
Systematic optimization of column chemistry, mobile phase, flow rate, and detection
Analysis & Reporting
High-throughput analysis with detailed chromatograms and compliant reports
UPLC vs HPLC
UPLC
- ✓ Particle Size: <2 µm (typically 1.7 µm)
- ✓ Operating Pressure: Up to 15000 psi
- ✓ Analysis Time: 2–10 minutes
- ✓ Resolution: Excellent (2–5× higher peak capacity)
- ✓ Sensitivity: 3–5× higher
HPLC
- ✗ Particle Size: 3–5 µm
- ✗ Operating Pressure: Up to 6000 psi
- ✗ Analysis Time: 15–60 minutes
- ✗ Resolution: Good
- ✗ Sensitivity: Standard
Capabilities & Services
- •Advanced UPLC Systems and sub-2 µm column technology (BEH, CSH, HSS)
- •Agilent 1290 Infinity II and Waters Acquity UPLC H-Class & I-Class Systems
- •PDA, UV-Vis, and Fluorescence detectors with binary/quaternary solvent delivery
Ready for High-Resolution Analysis?
State-of-the-art UPLC systems with sub-2 µm column technology for fast, compliant, and cost-effective solutions.
Extending Our Capacities With UPLC
Putting any advanced technology to good use is the USP of our genomics services. We deliver mission-critical, scalable solutions with UPLC.
Qualitative analysis for individual compounds
Quantitative analysis for trace amounts
Quantitation of polymer additives
Mapping of glycopeptides and peptides
Testing of impurities and surfactant analysis
Advanced UPLC Systems
- Agilent 1290 Infinity II UPLC System
- Waters Acquity UPLC H-Class & I-Class Systems
- Binary and Quaternary solvent delivery systems
- Photodiode Array (PDA), UV-Vis, Fluorescence detectors
- Sub-2 µm particle columns (BEH, CSH, HSS technology)
Key UPLC Services We Offer
Pharmaceutical Assay & Content Uniformity:
Impurity Profiling: Process, degradation, genotoxic impurities
Stability-Indicating Method Development: per ICH Q1A–Q1E
Full Analytical Validation: per ICH Q2(R1), USP, FDA guidelines
Bioanalytical Method Development: for pharmacokinetics