Microbiome Division

Metagenome Analysis

Unlock the complex world of microbial communities. Utilizing 16S/18S/ITS and Shotgun Metagenomics, we provide end-to-end bioinformatics answering "Who is there?" and "What are they doing?".

Analysis Capabilities

1. Taxonomic Diversity Profiling

Amplicon or Shotgun data classification (Phylum to Species).

Alpha Diversity: Chao1, Shannon, Simpson (Richness & Evenness).
Beta Diversity: PCoA, NMDS (Bray-Curtis, UniFrac).

2. Functional Pathway Analysis

Shotgun data mapped to reconstruct active biochemical pathways (Nitrogen, Carbon cycling, etc.) within the community.

3. AMR & Enzyme Discovery

Targeted screening against the Comprehensive Antibiotic Resistance Database (CARD) and mining for industrial biocatalysts.

Study Environments

Clinical Microbiome: Gut, skin, and oral microbiome profiling in relation to disease states (Dysbiosis).
Environmental: Soil, ocean, and extreme environment metagenomics for ecological monitoring.
Industrial/Agriculture: Bioremediation tracking, fermenter community dynamics, and plant rhizobiome analysis.

Instrument Compatibility

Amplicon Platforms

Illumina MiSeq (V2/V3), NextSeq 550, Thermo Fisher Ion S5, GeneMind GenoLab M.

Shotgun Metagenomics

Illumina NovaSeq X, MGI T7, Oxford Nanopore (GridION/PromethION) for real-time surveillance.

Data Quality & AI Pipeline

• 16S Data: 100k - 500k reads/sample, Q30 > 75%
• Shotgun Data: 5-10GB Raw Data/sample, Q30 > 80%
• AI Utilization: We apply Kraken2 ML-based algorithms for rapid taxonomic classification and MetaBat2 for predictive functional binning of MAGs (Metagenome-Assembled Genomes).

Tools: QIIME2, DADA2, HUMAnN3, MEGAHIT, MetaPhlAn3, SILVA, KEGG.

Service Tiers & Deliverables

Amplicon Profiling

16S / 18S / ITS Diversity Analysis

₹3,000 - ₹5,000

TAT: 10 - 15 Days

Deliverables: OTU/ASV Tables, Krona Charts, Alpha/Beta Diversity Plots, LEfSe Biomarker Discovery.

Shotgun Functional Analysis

Comprehensive Strain & Pathway Mapping

₹6,000 - ₹10,000

TAT: 3 - 4 Weeks

Deliverables: Deep Taxonomic Profiles, Functional Gene Counts (SEED/KEGG), AMR (CARD) & Virulence Factor Profiles.

Publication Support Included

Full metadata analysis integration, robust statistical validation (PERMANOVA/ANOSIM), methodology write-ups, and figure generation tailored for high-impact journals like Microbiome or ISME Journal.