LC-MS Analysis Services
LC-MS/MS for Impurity Profiling, Metabolites & Pharmacokinetics
What is LC-MS Analysis?
Liquid Chromatography-Mass Spectrometry (LC-MS) couples chromatographic separation with mass spectrometry detection for precise molecular weight determination, structural characterization, and quantification at trace levels (pg–ng/mL).
Key advantages: High sensitivity/specificity, ability to handle polar/non-polar compounds, and structural information from fragmentation patterns – far superior to HPLC-UV alone for unknowns and trace impurities.
Our LC-MS Capabilities & Instrumentation
End-to-End Analysis Workflow
Project Consultation
Free guidance on analyte properties, matrix, detection limits, and regulatory needs.
Sample Preparation & QC
Protein precipitation, SPE, LLE optimized for biofluids/API; internal standard addition.
Method Development
Column/mobile phase screening, gradient optimization, MRM transition selection.
Analysis & Validation
System suitability, calibration curves, full validation per FDA/ICH guidelines.
Data Processing & Reporting
Chromatogram review, quantification, impurity/metabolite identification.
Key Applications of Our LC-MS Analysis
- Pharmaceuticals – Process/genotoxic impurity profiling, forced degradation products
- Bioanalysis & Pharmacokinetics – Drug/metabolite quantification in plasma/serum
- Metabolite Identification – Phase I/II metabolites, reactive intermediates
- Herbal & Nutraceutical – Marker compounds, adulterants, bioavailability
- Clinical Research – Biomarker discovery, therapeutic drug monitoring
- Environmental/Food – Trace contaminants, pesticide residues
LC-MS Advantages & Limitations
- Ultra-high sensitivity (ppt–ppb range) & selectivity for trace analytes
- Structural elucidation via MS/MS fragmentation
- Robust for complex biological/pharma matrices
- Matrix effects/ion suppression require careful mitigation
- Higher cost than HPLC-UV (offset by superior data quality)
- Requires skilled method optimization
For UV-based quantification, see our HPLC Analysis. For volatile compounds, explore GC-MS.
Exclusive Applications
Our robust LC-MS system helps in cracking the most challenging applications in various fields such as:
Pharmaceutical industries.
Forensics department.
Environmental sample analysis.
Food & beverage industry.
Get Started with LC-MS Analysis in India
Need precise LC-MS/MS for impurity profiling, PK studies, or method validation? Contact Coimbatore’s trusted analytical lab for a personalized quote today.