Transcriptomics Division

Transcriptome Analysis (RNA-Seq)

Decode gene expression dynamics. We map the functional state of cells through rigorous statistical modeling, Differential Gene Expression (DGE), and AI-driven pathway interpretation.

Core Analyses

1. Differential Gene Expression (DGE)

Identifying statistically significant Up-regulated and Down-regulated genes across biological conditions, time-series, or developmental stages.

2. Functional Enrichment & Pathways

Contextualizing DGE results against Gene Ontology (GO), KEGG, and Reactome databases to highlight altered systemic biological pathways.

3. Advanced Structural Analysis

Alternative splicing detection, novel transcript discovery, and Gene Co-expression Network Analysis (WGCNA).

Application Areas

Microbial: Response to stress, antibiotic resistance gene expression, and Dual RNA-Seq.
Plant: Abiotic/biotic stress responses, crop yield traits, and developmental tissues.
Animal & Human: Biomarker discovery, cancer transcriptomics, immunology profiling, and toxicology.

Short-Read Platforms

Illumina (NextSeq 2000, NovaSeq X), MGI (DNBSEQ-G400), AVITI, Thermo Ion GeneStudio. Best for robust DGE.

Long-Read Platforms

PacBio (Iso-Seq), Oxford Nanopore (Direct RNA-Seq). Best for full-length isoform discovery.

AI Advantage

We utilize Predictive ML models for biomarker classification and LLM-driven synthesis to summarize complex biological pathways from highly enriched gene modules.

Data & Quality Requirements

• Format: De-multiplexed FASTQ with MD5 checksums
• Read Depth: 20-30 Million Reads/Sample (Eukaryotic), 5-10M (Microbial)
• Quality Metric: Q30 > 85%
• Sample Integrity: RNA Integrity Number (RIN) > 7.0 (via Agilent 2100)

Pipeline Tools

Alignment & Quant: STAR, HISAT2, Salmon, featureCounts

Statistical DGE: DESeq2, edgeR, limma-voom

Functional Mapping: clusterProfiler, g:Profiler, STRING

Commercials & Data Deliverables

Analysis Depth	TAT	Approx Price
Standard DGE Analysis	14 Days	₹8,000 - ₹15,000 / Sample
Advanced (Iso-Seq/Long Read)	21 Days	₹10,000 - ₹18,000 / Sample

Publication Support Included: Manuscript methodology sections and high-resolution figure preparation.

Final Outputs

Normalized TPM/FPKM

Comprehensive DGE Excel

High-Res Volcano & MA Plots

Clustered Heatmaps

GO/KEGG Bubble Charts

WGCNA Network Plots