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Primer Walking Services
Sequential primer-walking sequencing to resolve long inserts, plasmids and BAC clones beyond a single read length — with iterative primer design for complete, contiguous coverage.
What we offer
Long insert & plasmid sequencing
Iterative primer design
Complete contiguous coverage
Assembled consensus delivery
What you receive
Raw FASTQ data with QC reports
Reference-aligned BAM / CRAM files
Annotated variant calls (VCF)
De novo / hybrid assembly contigs
Coverage & quality metrics dashboard
Publication-ready summary report
Workflow
1
Sample & QC
Extraction, QC and library prep under documented, standardised protocols.
2
Sequencing / Processing
Calibrated runs and pipelines on validated platforms.
3
Bioinformatics
HPC-backed assembly, alignment, variant calling and annotation.
4
Report & Insight
Publication-ready reports, with a dedicated project manager throughout.
Frequently asked
We accept tissue, whole blood, cell pellets, extracted DNA/RNA and a range of environmental and microbial samples. Our team advises on collection and shipping for each project.
Specifications
PlatformsIllumina · PacBio · ONT
Read strategyShort / Long / Hybrid
CoverageProject-dependent
Sample inputDNA / RNA / Tissue
Turnaround2 – 6 weeks
Get a tailored quote
Tell us your sample type, scope and timelines — we'll respond within one business day.
Request a Quote